Laboratory Skills: Plate Count Examples. Solution Example 1. Three methods of how to calculate the amount of bacteria are illustrated.

You should use the method that makes the most sense to you. The dilution table looks as follows: Unknown. Volume Transferred. Dilutent Volume. Total Volume. Dilution A1 ml. 99. Dilution B1 ml. 99 ml. Dilution C1 ml. 9 ml.

Dilution D is used to inoculate two nutrient agar plates. The exponent for this problem can be determined by counting zeros. How To Install Aix Mod For Bf2. The total number of bacteria in the original sample is 3x. Solution Example 2. The dilution table looks as follows: Unknown. Volume Transferred.

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Dilutent Volume. Total Volume. Dilution A1. 0 ml. Dilution B1 ml. 5 ml. Dilution C1 ml. 99. Dilution D1 ml. 9 ml. Dilution D is used to inoculate two nutrient agar plates. However, 1. 0x. 10.

TABLE OF CONTENTS. Fecal Coliform. Section 1: General 2. Section 2: Introduction to Fecal Coliform Testing 2. Section 3: Glossary 2-3. Section 4: Approved Methods 3.

Convert 1. 0x. 10. Therefore, the number of bacteria in the original solution is 1x. Method 2. Calculate the ratios of the Total Volume / Volume Transferred and then multiply it by the colonies counted on the plate. Dilution A) = 2. 0/1. Dilution B) 6/1 = 6. Dilution C) = 1. 00.

Rounds to 1. 00,0. Number of bacteria in the original solution. Method 3 - EASY Math. To use this solution three criteria must be met: the number of bacteria colonies on the plate must be between 1. There is no easy math for this solution because dilutions A & B were not done as 1 to 9, 1 to 9.